Derece Alan Üniversite Yıl Moleküler Biyoloji ve Genetik Bölümü

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1 ÖZGEÇMİŞ VE ESERLER LİSTESİ 1. Adı Soyadı: Pınar Obakan İletişim Bilgileri: Adres: İstanbul Kültür Üniversitesi, Fen-Edebiyat Fakültesi, Moleküler Biyoloji ve Genetik Bölümü, MOBIGEN Bakırköy-İstanbul Telefon: Mail: p.obakan@iku.edu.tr 2. Doğum Tarihi: Ünvanı: Yrd. Doç. Dr. 4. Öğrenim Durumu: Derece Alan Üniversite Yıl Lisans Biyoloji Istanbul Universitesi, Türkiye 2007 Y. Lisans Moleküler Biyoloji ve Genetik Université Paul Sabatier, Toulouse III, 2008 Fransa Doktora Biyoloji Istanbul Universitesi, Türkiye Akademik Ünvanlar Derece Alan Üniversite Yıl Biyolog Araş. Gör. Yard. Doç. Dr. Moleküler Biyoloji ve Genetik Bölümü Moleküler Biyoloji ve Genetik Bölümü Moleküler Biyoloji ve Genetik Bölümü İstanbul Kültür Üniversitesi İstanbul Kültür Üniversitesi İstanbul Kültür Üniversitesi Yönetilen Yüksek Lisans ve Doktora Tezleri 6.1. Yüksek Lisans Tezleri Ephrin B1 Reverse Signaling: From Cell Surface to Nucleus Université Paul Sabatier, Toulouse III, Tez Danışmanı: Dr. Alice Davy 6.2. Doktora Tezleri Farklı Prostat Kanseri Hücre Hatlarında Epibrassinolidle Tetiklenen Apoptotik Süreçte Poliaminlerin Rollerinin Araştırılması, Istanbul Universitesi, Tez Danışmanları: Prof. Dr. Narçın Palavan Ünsal ve Prof. Dr. Sehnaz Bolkent 1

2 7. Yayınlar 7.1. Uluslararası hakemli dergilerde yayınlanan makaleler (SCI & SSCI & Arts and Humanities) 1. E. D. Arisan, P. Obakan, A. Çoker-Gürkan, A. Calcabrini, E. Agostinelli, N. Palavan Ünsal, CDK inhibitors induce mitochondria-mediated apoptosis through the activation of polyamine catabolic pathway in LNCaP, DU145 and PC3 prostate cancer cells. Current Pharmaceutical Design, 20: (9), 2014 (Araştırma Makalesi) 2. Ozfiliz P, Arisan ED, Coker A, Obakan P, Eralp T, Doganay-Dinler G, Palavan-Unsal N, Bag-1L mediated chemoresistance mechanism through preventing downregulation of Mcl-1 and c-raf by heat shock proteins in HeLa cells, Asian Pacific Journal of Cancer Prevention, 2014 (Baskıya kabul edildi) 3. Elif Damla Arisan, Yunus Akkoç*, Kaan Gencer Akyüz*, Ezgi Melek Kerman*, Pinar Obakan, Ajda Çoker-Gürkan, Narçin Palavan Ünsal, Polyamines modulate roscovitine-induced cell death decision as autophagy or apoptosis in MCF-7 and MDA-MB-231 breast cancer cells, Molecular Medicine Reports, (Baskıya kabul edildi; Baskıda). 4. Ajda Çoker-Gürkan, Elif Damla Arisan, Pinar Obakan, Pelin Özfiliz, Betsi Köse, Güven Bıçkıcı, Narçin Palavan Ünsal, Roscovitine finalized autophagy later than apoptosis by downregulating of Bcl-2 in HeLa cells, Molecular Medicine Reports, (Baskıya kabul edildi). 5. P. Obakan, P. Ozfiliz, E.D. Arısan, A. Çoker-Gürkan, N. Palavan Ünsal, Purvalanol A is a strong apoptotic inducer via activating polyamine catabolic pathway in MCF-7 estrogen receptor positive breast cancer cells, Molecular Biology Reports, 2013, DOI: /s (Araştırma Makalesi). 6. P.Obakan, E.D. Arisan, A. Calcabrini, E. Agostinelli, S. Bolkent, N. Palavan-Unsal. Activation of Polyamine Catabolic Enzymes Involved in Diverse Responses Against Epibrassinolide-induced Apoptosis in LNCaP and DU145 Prostate Cancer Cell Lines, Amino Acids, DOI: /s (Araştırma Makalesi) 7. Çoker-Gurkan, E. D. Arisan, P. Obakan, N. Palavan-Ünsal, Inhibition of polyamine oxidase prevented cyclin dependent kinase inhibitor-induced apoptosis in HCT116 colon carcinoma cells Apoptosis, /s (Araştırma Makalesi) 8. E. D. Arısan, A.Coker, P.Obakan, N.Palavan-Unsal, "Inhibition of ornithine decarboxylase altered the roscovitine-induced mitochondria mediated apoptosis in MCF-7 breast cancer cells", Molecular Medicine Reports (ISI), pp., 2012, DOI: /mmr (Araştırma Makalesi) 7.2. Uluslararası diğer hakemli dergilerde yayınlanan makaleler 7.3. Uluslararası bilimsel toplantılarda sunulan ve bildiri kitabında (Proceedings) basılan bildiriler 2

3 1. P. Obakan, E.D. Arisan, S. Bolkent, N. Palavan-Unsal. Epibrassinolide Induces Apoptosis by Targetting Polyamine Catabolism in LNCaP Prostate Cancer Cells, First International Congress of the Molecular Biology Association of Turkey, November 2012, Istanbul-TURKEY. (Poster sunum: P. Obakan) 2. O. Berrak, Y. Akkoc, P. Obakan, E.D. Arisan, A. Coker-Gurkan, N. Palavan-Unsal. Effects of CDK Inhibitors and Rapamycin Combination on Autophagy With The Status of AR expression on Androgen Dependent LNCaP and Androgen Independent DU145 Prostate Cancer Cells, First International Congress of the Molecular Biology Association of Turkey, November 2012, Istanbul-TURKEY. (Poster sunum: O.Berrak) 3. D. Coskun, P. Obakan, E.D. Arisan, A. Coker-Gurkan, N. Palavan-Unsal. Epibrassinolide Induced Apoptotic Cell Death in HCT116 and HT29 Colon Carcinoma cells, First International Congress of the Molecular Biology Association of Turkey, November 2012, Istanbul-TURKEY. (Poster sunum: D. Coskun) 4. Y. Akkoc, E.D. Arisan, A. Coker-Gurkan, P. Obakan, N. Palavan-Unsal. Understanding the Role of Bcl-2 in Curcumin induced Autophagic/Apoptotic Cell Death Mechanism in MCF-7 and MDA-MB- 231 Breast Cancer Cells, First International Congress of the Molecular Biology Association of Turkey, November 2012, Istanbul-TURKEY. (Poster sunum: Y. Akkoc) 5. H. Ilhan, A. Coker-Gurkan, E.D. Arisan, P. Obakan, N. Palavan Unsal. N. Diethylnorspermine (DENSpm) Induced Apoptosis in SW480 Colon Cancer Cells, First International Congress of the Molecular Biology Association of Turkey, November 2012, Istanbul-TURKEY. (Poster sunum: H. Ilhan) 6. P. Obakan, E.D. Arisan, A. Calcabrini, E. Agostinelli, S. Bolkent, N. Palavan-Unsal. Epibrassinolide Targets Polyamine Catabolism and Triggers Apoptosis in LNCaP, DU145 and PC3 Cell Lines. International Congress on Polyamines: Biological and Clinical Perspectives, 2-7 September 2012, Istanbul-TURKEY. (Seçili poster sözlü sunum: P.Obakan) 7. S. Akyuz, T. Akyuz, P. Obakan, E.D. Arisan, S. Bolkent, N. Palavan-Unsal. FTIR Spectroscopic Investigation of The Effects of Polyamine Oxidase Inhibitor MDL on The Interaction of Epibrassinolide With Cultured LNCaP Prostate Cancer Cells. International Congress on Polyamines: Biological and Clinical Perspectives, 2-7 September 2012, Istanbul-TURKEY. (Poster sunum: S. Akyuz) 8. S. Yildirim, M.B. Ozturk, E.D. Arisan, P. Obakan, A. Coker-Gurkan, N. Palavan-Unsal. PPARγ is the Critical Upstream Factor in Cyclin-Dependent Kinase Inhibitors Roscovitine and Purvalanol A- Induced SSAT Upregulation in MCF-7 Cells. International Congress on Polyamines: Biological and Clinical Perspectives, 2-7 September 2012, Istanbul-TURKEY. (Poster sunum: S. Yildirim ve M.B. Ozturk) 3

4 9. A. Coker-Gurkan, P. Obakan, E.D. Arisan, A. Calcabrini, E. Agostinelli, N. Palavan-Unsal. The Effect of Polyamine Catabolism Products on CDK Inhibitors-Induced on HCT116 wt and Bax -/- Colon Carcinoma Apoptotic Cell Death. International Congress on Polyamines: Biological and Clinical Perspectives, 2-7 September 2012, Istanbul-TURKEY. (Seçili poster sözlü sunum: A. Coker- Gurkan) 10. B. Kose, G. Alkurt, A. Coker-Gurkan, E.D. Arisan, P. Obakan, N. Palavan-Unsal. ODC is a Mediator of the Purvalanol A-Induced Apoptotic Signaling via the p38 Mitogen-Activated Protein Kinase Pathway in MCF-7 Breast Cancer Cells. International Congress on Polyamines: Biological and Clinical Perspectives, 2-7 September 2012, Istanbul-TURKEY. (Poster sunum: B. Kose ve G. Alkurt) 11. D. Coskun, P. Obakan, E.D. Arisan, A. Coker-Gurkan, N. Palavan-Unsal. The Potential Role of Polyamines in EBR-Induced Apoptotic Cell Death in HCT116 Colon Carcinoma Cells. International Congress on Polyamines: Biological and Clinical Perspectives, 2-7 September 2012, Istanbul- TURKEY. (Poster sunum: D. Coskun) 12. H. Ilhan, A. Coker-Gurkan, P. Obakan, E.D. Arisan, N. Palavan-Unsal. Timing is Critical in the Decision of Apoptosis or Autophagy by N1, N11-Diethylnorspermine (DENSpm) treatment in HCT116 Colon Cancer Cells. International Congress on Polyamines: Biological and Clinical Perspectives, 2-7 September 2012, Istanbul-TURKEY. (Poster sunum: H. Ilhan) 13. O. Irmak, E.D. Arisan, A. Coker-Gurkan, P. Obakan, N. Palavan-Unsal. The Role of Polyamine Catabolic Enzymes in Cisplatin-Induced Apoptotic Cell Death in MCF-7 Cells. International Congress on Polyamines: Biological and Clinical Perspectives, 2-7 September 2012, Istanbul- TURKEY. (Poster sunum: O. Irmak) 14. E. D. Arisan, O. Berrak, P. Obakan, N. Palavan-Unsal. Cyclin Dependent Kinases Exert Their Apoptotic Potential By Acting on mtormediated Energy Signaling Cascade In The Presence Of Activated Polyamine Catabolism in LNCap, DU145 and PC3 Prostate Cancer Cells. International Congress on Polyamines: Biological and Clinical Perspectives, 2-7 September 2012, Istanbul- TURKEY. (Sözlü sunum: E.D. Arisan) 15. Y. Akkoc, M. Celik, P. Obakan, A. Coker-Gurkan, E.D. Arisan, N. Palavan-Unsal. The Functional Role of Bcl-2 in the Regulation of Curcumin-Induced Autophagy/ Apoptotic Cell Death in MCF-7 Breast Cancer Cells. Apoptosis and Cancer, June 2012,Cambridge- UK. (Poster sunum:y. Akkoc ve M. Celik) 16. P. Obakan, E.D. Arisan, S. Bolkent, N. Palavan-Unsal. 24-Epibrassinolide Induces SSAT and Causes Caspase-Dependent Apoptosis In Different Prostate Cancer Cell Lines. Biogenic Amines: Biochemical, Physiological and Clinical Perspectives, September 21 25, 2011 Alberè di Tenna (Trento), ITALY. (Seçili poster sözlü sunum: P. Obakan) 4

5 17. N. Palavan-Unsal, E.D. Arisan, P. Obakan, A. Coker, I. Ozdemir. Cyclin Dependent Kinase Inhibitors Modulates Polyamine Metabolism in MDA-MB-231 Cells, 2nd International Conference on the Role of Polyamines and their Analogs in Cancer and other Diseases, 1-6 December, 2010, Sapienza University of Roma, ITALY. (Sözlü sunum: N. Palavan-Unsal) 18. E.D. Arisan, P. Obakan, A. Coker, N. Palavan-Unsal. Investigation Of Purvalanol-Induced Apoptosis In MCF-7 Breast Cancer Cell Lines. 2nd International Conference on the Role of Polyamines and their Analogs in Cancer and other Diseases, 1-6 December, 2010, Sapienza University of Roma, ITALY. (Poster sunum: E.D. Arisan) 19. P. Obakan, E.D. Arisan, N. Palavan-Unsal, A. Coker. Combined Treatment of Roscovitine and DFMO Induced Caspase Dependent Apoptosis in MCF-7 cells. 2nd International Conference on the Role of Polyamines and their Analogs in Cancer and other Diseases, 1-6 December, 2010, Sapienza University of Roma, ITALY. (Sözlü sunum: P.Obakan) 20. E.D. Arisan, N. Palavan-Unsal, P. Obakan. Investigation of Roscovitine (CYC202)-Induced Apoptosis Related With Polyamine Metabolism in MCF-7 Human Breast Cancer Cell Line. IUBMB LIFE, 61: 370, III. Congress of Molecular Medicine, From Cell to Bedside, 5-8 May 2009, Harbiye Military Museum, Istanbul, Turkey. (Poster sunum: P.Obakan) 7.4. Yazılan uluslararası kitaplar veya kitaplarda bölümler 7.5. Ulusal hakemli dergilerde yayınlanan makaleler 7.6. Ulusal bilimsel toplantılarda sunulan ve bildiri kitabında basılan bildiriler 7.7. Diğer yayınlar 1. P. Obakan, E.D. Arisan, S. Bolkent, N. Palavan-Unsal. Understanding the roles of polyamines in EBR-induced autophagy, Gordon Research Conferences: Polyamines, Regulation and Role of Polyamines in Biology and Disease, June 16-21, 2013, Waterville Valley, NH, USA, and Gordon Research Seminars: Polyamines in Biology and Disease, June 15-16, 2013, Waterville Valley, NH, USA. (Sözlü sunum: P.Obakan) 2. O. Berrak, E.D. Arisan, P. Obakan, A. Coker-Gurkan, N. Palavan-Unsal. Co-treatment of rapamycin prevented activation of polyamine oxidase and apoptosis via triggering autophagy after CDK inhibitor treatment in LNCaP cells, Gordon Research Conferences: Polyamines, Regulation and Role of Polyamines in Biology and Disease, June 16-21, 2013, Waterville Valley, NH, USA. (Poster sunum: O. Berrak) 3. Y. Akkoc, E.D. Arisan, P. Obakan, A. Coker-Gurkan, N. Palavan-Unsal. N. Co-treatment of PI3K inhibitor and curcumin overcomes Bcl-2 mediated drug-resistance in MCF-7 breast cancer cells via activating SSAT, Gordon Research Conferences: Polyamines, Regulation and Role of Polyamines in Biology and Disease, June 16-21, 2013, Waterville Valley, NH, USA. (Poster sunum: Y. Akkoc) 5

6 4. D. Coskun, P. Obakan, E.D. Arisan, A. Coker-Gurkan, N. Palavan-Unsal. The Potential Role of Polyamines in Epibrassinolide-Induced Apoptotic Cell Death in HCT116 and HT29 Colon Carcinoma Cells, Gordon Research Conferences: Polyamines, Regulation and Role of Polyamines in Biology and Disease, June 16-21, 2013, Waterville Valley, NH, USA. (Poster sunum: N. Palavan-Unsal) 5. Coker-Gurkan, E.D. Arisan, H. Ilhan, P. Obakan, N. Palavan-Unsal. Inhibition of Cellular Autophagic Responses against N1, N11-Diethylnorspermine (DENSpm) Enhanced Apoptosis in HCT116 and SW480 Colon Cancer Cells, Gordon Research Conferences: Polyamines, Regulation and Role of Polyamines in Biology and Disease, June 16-21, 2013, Waterville Valley, NH, USA (Poster sunum: A. Coker-Gurkan) 6. O. Berrak, E.D. Arisan, P. Obakan, N. Palavan-Unsal. Investigation Of Possible Role of mtor in CDK Inhibitors Induced Apoptotic Cell Death in LNCaP, DU145 and PC3 Prostate Cancer Cell Lines. Graduate Student Immunology Congress, April 27-28, 2012, Harvard University, Boston, USA. (Poster sunum: O. Berrak) 7. P. Obakan, P. Ozfiliz, E.D. Arisan, A. Coker-Gurkan, N. Palavan-Unsal. Purvalanol A is a Strong Apoptotic Inducer via Activating Polyamine Catabolic Pathway in MCF-7 Estrogen Receptor + Breast Cancer Cells. EMBO Young Scientist Forum, June 2012, Istanbul-TURKEY. 8. P.Obakan, E.D.Arisan, S.Bolkent, N.Palavan-Unsal. 24-Epibrassinolide induced apoptosis by modulating polyamine catabolic pathway in various prostate cancer cell lines. Gordon Research Conferences on Polyamines. Polyamine Genetics, Metabolism, Cellular Homeostasis and Drug Discovery. June Waterville Valley, USA, 2011 (Poster sunum: P. Obakan) 9. E.D.Arisan, P.Obakan, N.Palavan-Unsal. Roscovitine induced apoptosis and activated polyamine catabolic pathway in LNCap, DU145 and PC3 prostate cancer cell lines. Gordon Research Conferences on Polyamines. Polyamine Genetics, Metabolism, Cellular Homeostasis and Drug Discovery. June 19-24, Waterville Valley, USA, 2011 (Sözlü sunum: E. D. Arisan) 8. Ulusal & Uluslararası Projeler Epibrassinolidle Tetiklenen Hücre Ölümünün Endoplazmik Retikulum Stresi İle İlişkisinin Kolon Kanseri Hücre Hatlarında Araştırılması (3001-TÜBİTAK Başlangıç Ar-Ge Projeleri Destekleme Programı, 113Z845, , Yürütücü) Curcumin in terapotik etkinliğinin otokrin büyüme hormonu sinyal yolağı aracılığı ile farklı meme kanseri hücrelerinde poliamin metabolizması irdelenerek incelenmesi (1001-TÜBİTAK KBAG- Proje No: 113Z791, , Araştırmacı) Kolon Karsinoma Hücrelerinde Epibrassinolid Tarafından Tetiklenen Apoptotik Süreçte Poliaminlerin Rolü (TÜBITAK, Hızlı Destek Projesi, 212T168, Bursiyer) 6

7 Farklı Prostat Kanseri Hücre Hatlarında Epibrassinolidle Tetiklenen Apoptotik Süreçte Poliaminlerin Rollerinin Araştırılması (Doktora Tezi, İstanbul Üniversitesi BAP Projesi, 20958, , Yürütücü) CDK Inhibitörü Roskovitin Tarafından Tetiklenen Apoptotik Yolakta Poliamin Katabolizmasının Rolünün LNCaP, DU145 Ve PC3 Prostat Kanseri Hücre Hatlarında İncelenmesi (TÜBITAK, Hızlı Destek Projesi, 210T107, Bursiyer) Cdk Inhibitörü Purvalanolün (CYC 202) Meme Kanseri Hücrelerindeki (MCF-7) Etkisinin Apoptotik Sinyal Molekülleri ve Poliamin Parametreleri Ile Araştırılması (İstanbul Kültür Üniversitesi, Arastırmacı, ) Cdk Inhibitörü Roskovitinin (CYC 202) Meme Kanseri Hücrelerindeki (MCF-7) Etkisinin Apoptotik Sinyal Molekülleri ve Poliamin Parametreleri ile Araştırılması (İstanbul Kültür Üniversitesi, Araştırmacı, ) 9. Idari Görevler 10. Bilimsel ve Mesleki Kuruluslara Üyelikler 2009: Biochemical Society 2010: Moleküler Kanser Arastırma Derneği (MOKAD) 2010: European Association for Cancer Research (EACR) 2012: Moleküler Biyoloji Derneği (MBD) 11. Ödüller ve Burslar Türk Eğitim Vakfı (TEV)- Fransa Büyükelçiliği Müsterek Fransa Bursu (Yüksek Lisans, ) İstanbul Kültür Üniversiesi Akademik Başarı Ödülü, 2009 Roma La Sapienza Üniversitesi Erasmus Öğretim Elemanı hareketliliği programı (Eylül, 2011) Biogenic Amines: Biochemical, Physiological and Clinical Perspectives, September 21 25, 2011 Alberè di Tenna (Trento), ITALY, Katılım Bursu (Eylül, 2011) Gordon Research Seminars: Polyamines in Biology and Disease, June 15-16, 2013, Waterville Valley, NH, USA. Katılım Bursu (Haziran, 2013) İstanbul Kültür Üniversiesi Akademik Başarı Ödülü (Kasım, 2013) 12. Etkinlikler: 1. Yurt dışında gerçekleştirilen akademik faaliyetler: Temple University, School of Medecine, Fels Institute of Cancer Research and Molecular Biology, Philadelphia, Ziyaretçi bilim adamı, ABD 2013 ERASMUS değişim programı, Roma La Sapienza Üniversitesi, İtalya

8 2. Düzenlenen bilimsel etkinlikler: Organize Edilen Uluslararası Kongreler Polyamines: Biological and Clinical Perspectives: INTERNATIONAL POLYAMINE CONGRESS, 2-7 Eylül 2012, (Kongre Organizasyon Komite Üyesi). Organize Edilen Bilimsel Toplantılar Genomik Uygulamalar, MOBİGEN DNA OKULU, Sertifika Programı, Eylül 2008, (Organizasyon Komite Üyesi). DNA Hasarı, Onarımı ve Hastalıklarla İlişkisi, MOBİGEN DNA OKULU, Nisan 2013, (Organizasyon Komite Üyesi) Nanotechnology and Tissue Engineering: Current Challenges and Future Prospects, Bilimsel Toplantı MOBİGEN, Harvard Üniversitesi ve Massachussets Institute of Technology (MIT) İşbirliği, Aralık 2013, (Organizasyon Komite Üyesi) 3. Katıldığı Uluslararası Bilimsel Etkinlikler: Gordon Research Seminars: Polyamines in Biology and Disease, June 15-16, 2013, Waterville Valley, NH, USA. Gordon Research Conferences: Polyamines, Regulation and Role of Polyamines in Biology and Disease, June 16-21, 2013, Waterville Valley, NH, USA. First International Congress of the Molecular Biology Association of Turkey, November 2012, Istanbul-TURKEY. International Congress on Polyamines: Clinical and Biological Perspectives, September 2-7, 2012, Istanbul- TURKEY. EMBO Young Scientist Forum, June 2012, Istanbul-TURKEY. Biogenic Amines: Biochemical, Physiological and Clinical Perspectives, September 21-25, 2011 Alberè di Tenna (Trento), ITALY. Gordon Research Conferences on Polyamines. June 19-24, 2011, Waterville Valley,USA. Gordon Reseacrh Seminars on Polyamines. June 18-19, 2011, Waterville Valley,USA 2nd International Conference on the Role of Polyamines and their Analogs in Cancer and other Diseases, 1-6 December, 2010, Sapienza University of Roma, ITALY. III. Congress of Molecular Medicine, From Cell to Bedside, 5-8 May 2009, Harbiye Military Museum, Istanbul, Turkey. 8

9 13. Son iki yılda verdiği lisans ve lisansüstü düzeyindeki dersler: Akademik Yıl Dönem Dersin Adı Haftalık Saati Öğrenci Teorik Uygulama Sayısı Güz Sinir Bilimi Moleküler Biyoloji Teknikleri I Bahar Moleküler Mikrobiyoloji Laboratuvarı Hayvan Doku Kültürü Enzimoloji Moleküler Biyoloji Teknikleri II