CV and PUBLICATION LIST. Degree Department University Year

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1 CV and PUBLICATION LIST CV Name/Surname: Ozkan DEMIRBAS Birth of Date: April 27, 1976 EDUCATION: Degree Department University Year Graduate Chemistry Education Balikesir University 1998 Master Chemistry (Physical) Balikesir University 2001 PhD Chemistry (Physical) Balikesir University 2006 Title of Master Thesis and Supervisor: Adsorption of Victoria Blue onto Perlite Surface (Supervisor: Prof. Dr. Mahir ALKAN) Title of PhD Thesis and Supervisors : Immobilization of Some Biomolecules onto Clay Minerals and Electrokinetic Properties (Supervisors: Prof. Dr. Mahir ALKAN, Assoc. Prof. Dr. Mehmet DOĞAN) Positions: Title Department Year Res. Assist. Balikesir University Faculty of Education Res. Assist. Balikesir University Faculty of Science and Literature Assist. Prof. Dr. Balikesir University Faculty of Science and Literature Master Students: Karadağ, A., Balikesir University, Institute of Natural and Applied Science. PhD Students:

2 Our Projects: 1. Rheology of Perlite and Boron Minerals and Investigation of Useable Industry, DPT Project Removal of Metal Ions from Aqueous Solutions by Modified Sepiolite Samples, TUBITAK Project, TBAG-2455 (104T067, 2004). 3. Investigation of Extrusion properties of perlite Balikesir University Research Found, (2006/19). 4. Investigation of Rheological Properties of Sepiolite Pastes Balikesir University Research Found, (2006/18). 5. Immobilization of Some Biomolecules onto Clay Minerals and Electrokinetic Properties, Balikesir University Research Found, (2004/17). 6. Removal of Dyes from Aqueous Solutions, Balikesir University Research Found, (2004/19). 7. Adsorption of Metal Ions from Aqueous Solutions, Balikesir University Research Found, (2004/16). Admin. Members: Awards: Courses Thought: Graduate Year Term Lesson I Hour (weekly) Theory Practice Research Seminar 2 6 Biochemistry Lab. I Number of student II Quantum Chemistry Colloid Chemistry 2 90 Physical Chem. Lab I 4 45 Research Seminar 2 6

3 WORKS A. PAPERS (SCI): A1. Demirbaş, Ö., Alkan, M., and Doğan, M., The Removal of Victoria Blue from Aqueous Solution by Adsorption on a Low-Cost Material, Adsorption. 8 (2002) A2. Alkan, M., Demirbaş, Ö., Doğan, M., Removal of Acid Yellow 49 from Aqueous Solution by Adsorption, Fresenius Environmental Bulletin. 13(11a) (2004) A3. Alkan, M., Çelikçapa, S., Demirbaş, Ö., Doğan, M., Sorption of Acid Red 57 from Aqueous Solution onto Sepiolite, Journal of Hazardous Materials. B1(16) (2004) A4. Alkan, M., Demirbaş, Ö., Çelikçapa, S., Doğan, M. Removal of Reactive Blue 221 and Acid Blue 62 Anionic Dyes from Aqueous Solutions by Sepiolite, Dyes and Pigments. 65 (2005) A5. Alkan, M., Karadaş, M., Doğan, M., Demirbaş, Ö., Zeta potentials of perlite samples in various electrolyte and surfactant media, Colloids and Surfaces, A: Physiochem. and Eng. Asp. 259 (2005) A6. Alkan, M., Karadaş, M., Doğan, M., Demirbaş, Ö., Adsorption of CTAB onto Perlite Samples from Aqueous Solutions, Journal of Colloid and Interface Science. 281 (2005) A7. Tekin, N., Demirbaş, Ö., Alkan, M., Adsorption of Cationic Polyacrylamide onto Kaolinite, Microporous and Mesoporous Materials. 85 (3) (2005) A8. Alkan, M., Demirbaş, Ö., Doğan, M., Electrokinetic Properties of Sepiolite Suspensions in Different Electrolyte Media, Journal of Colloid and Interface Science. 281 (2005) A9. Alkan, M., Demirbaş, Ö., Doğan, M., Electrokinetic Properties of Kaolinite in Mono- and Multivalent Electrolyte Solutions, Microporous and Mesoporous Materials. 83 (2005)

4 A10. Alkan, M., Demirbaş, Ö., Doğan, M.,, Zeta Potential of Unexpanded and Expanded Perlite Samples in Various Electrolyte Media, Microporous and Mesoporous Materials. 84 (2005) A11. Tekin, N., Dinçer, A., Demirbaş, Ö., and Alkan, M., Adsorption of Cationic Polyacrylamide onto Sepiolite, Journal of Hazardous Materials. 134 (1-3) (2006) A12. Tekin, N., Kadıncı, E., Demirbaş, Ö., Alkan, M., and Kara, A., Adsorption of Polyvinylimidazole onto Kaolinite, Journal of Colloid and Interface Science. 296 (2): (2006) A13. Tekin, N., Kadıncı, E., Demirbaş, Ö., Alkan, M., Kara, A., and Doğan, M., Surface Properties of Poly(vinylimidazole)-adsorbed Expanded Perlite, Microporous and Mesoporous Materias. 93 (1-3) (2006) A14. Doğan, M., Alkan, M., Demirbaş, Ö., Özdemir Y., and Özmetin, C., Adsorption Kinetics of Maxilon Blue GRL onto Sepiolite from Aqueous Solutions, Chemical Engineering Journal. 124 (2006) A15. Alkan, M., Demirbaş, Ö., Doğan, M., and Arslan, O., Surface Properties of Bovine Serum Albumin Adsorbed Oxides: Adsorption, Adsorption Kinetics and Electrokinetic Properties, Microporous and Mesoporous Materials. 96 (2006) A16. Alkan, M., Demirbaş, Ö., Doğan, M.,Adsorption kinetics and thermodynamics of an anionic dye onto sepiolite, Microporous & Mesoporous Materials. (2007) in press. B. International Presentations (Proceedings): B1. Alkan, M., Demirbaş, Ö., Doğan, M., Electrokinetic Properties of Sepiolite Suspensions in Heavy Metal Solutions, 4th International Aegean Analytical Chemistry Days, 29 September-3 October 2004, Kuşadası, Aydın-TURKEY. C. Chapters :

5 D. Papers (National) : E. National Presentations (Proceedings): E1. İlten, N., Alkan, M., Demirbaş, Ö., Prinanın Yakıt Olarak Değerlendirilmesi, V. Ulusal Yanma ve Hava Kirliliği Kontrolü Sempozyumu, Haziran 2000, Fırat Üniv.,Elazığ. F. Other Presentations (Abstract) : International [1] Alkan, M., Karadaş, M., Demirbaş, Ö., Doğan, M., Electrokinetic Behaviour of Perlite Suspensions, 4th International Aegean Analytical Chemistry Days, 29 September-3 October 2004, Kuşadası, Aydın-Turkey. [2] Alkan, M., Karadaş, M., Demirbaş, Ö., Doğan, M., Adsorption of CTAB onto Perlite Samples from Aqueous Solutions, 2nd International Aegean Physical Chemistry Days, 7-10 October 2004, Ayvalik, Balikesir-Turkey [3] Alkan, M., Demirbaş, Ö., Doğan, M., Potential Determining Ions at the Perlite/Electrolyte Interface and the Role of the Some Heavy Metal Cations, 2nd International Aegean Physical Chem.Days, 7-10 October 2004, Ayvalik, Balikesir- Turkey [4] Alkan, M., Demirbaş, Ö., Doğan, M., Zeta Potential Measurements at The Interfaces of The Kaolinite/Different Electrolyte Solutions, 2nd International Aegean Physical Chemistry Days, 7-10 October 2004, Ayvalik, Balikesir-Turkey [5] Alkan, M., Demirbaş, Ö., Doğan, M., Adsorption of Bovine Serum Albumin onto Some Clay Minerals and Electrokinetic Properties, 2nd International Aegean Physical Chemistry Days, 7-10 October 2004, Ayvalik, Balikesir-Turkey [6] Alkan, M., Demirbaş, Ö., Doğan, M., and Özdemir, Y., Kinetics and Mechanism of Removal of Maxilon Blue GRL by Adsorption onto Sepiolite, 2nd International Aegean Physical Chemistry Days, 7-10 October 2004, Ayvalik, Balikesir-Turkey [7] Doğan, M., Türkyılmaz, A., Alkan, M., Demirbaş, Ö., ph-dependence of Zeta Potential of Various Sepiolite Suspensions, 2nd International Aegean Physical Chemistry Days, 7-10 October 2004, Ayvalik, Balikesir-Turkey [8] Tekin, N., Demirbaş, Ö., Alkan, M., Adsorption of Cationic Polyacrylamide onto Kaolinite, X. European Ecological Congress - Eureco 2005, 8-13 November 2005,

6 Kuşadası, İzmir. [9] Demirbaş, Ö., Hopa, Ç., Alkan, M., and Doğan, M., Removal of Some Textile Dyes from Waste Water, X. European Ecological Congress - Eureco 2005, 8-13 November 2005, Kuşadası, İzmir. National [1] Demirbaş, Ö., Alkan, M., Doğan, M., Sulu Çözeltilerden Viktorya Mavisinin Perlit Örnekleri Yüzeyine Adsorpsiyonu, XV. Ulusal Kimya Kongresi, 2001, Boğaziçi Üniversitesi, İstanbul. [2] Demirbaş, Ö., Alkan, M., Doğan, M., Dursunbey Kömüründe Pritik Kükürt Miktarı ve Kömürden Kükürtün Uzaklaştırılmasına Ait Yöntemler İle Çevre Ilişkisinin Internet Ortamında Gösterimi, XV. Ulusal Kimya Kongresi, 2001, Boğaziçi Üni., İstanbul. [3] Doğan, M., Alkan, M., Demirbaş, Ö., Sulu Çözeltilerden Perlit Yüzeyine Metilen Mavisinin Adsorpsiyon Kinetiği ve Mekanizması, 1.Ege Fizikokimya Günleri, 5-7 Haziran 2002, Ege Üniversitesi, İzmir. [4] Alkan, M., Demirbaş, Ö., Çelikçapa, S., Tekstil Atık Sularından Acid Blue 62 nin Giderimi, XVII. Ulusal Kimya Kongresi, 2003, İstanbul Üniversitesi, İstanbul. [5] Alkan, M., Demirbaş, Ö., Çelikçapa, S., Sepiyolit Yüzeyine Acid Red 57 Adsorpsiyonu, XVII. Ulusal Kimya Kongresi, 2003, İstanbul Üniversitesi, İstanbul. [6] Alkan, M., Demirbaş, Ö., Çelikçapa, S., Sulu Çözeltilerden Acid Yellow 49 un Sepiyolit Yüzeyine Adsorpsiyonu, XVII. Ulusal Kimya Kongresi, 2003, İstanbul Üniversitesi, İstanbul. [7] Alkan, M., Demirbaş, Ö., Çelikçapa, S., Reactive Blue 221 in Sepiyolit Yüzeyine Adsorpsiyonu, XVII. Ulusal Kimya Kongresi, 2003, İstanbul Üniversitesi, İstanbul. [8] Alkan, M., Demirbaş, Ö., Doğan, M., Perlit Yüzeyine Kazein Adsorpsiyonu Ve Elektrokinetik Özellikleri, XIX. Ulusal Kimya Kongresi, 2005, Ege Üniversitesi, İzmir. [9] Alkan, M., Kalay, B., Demirbaş, Ö., Doğan, M., Asit Aktive Edilmiş Kil Örnekleri İle Sulu Çözeltilerden Fenol Giderimi, XIX. Ulusal Kimya Kongresi, 2005, Ege Üniversitesi, İzmir. [10] Tekin, N., Kadıncı, E., Demirbaş, Ö., Alkan, M., Kara A., and Beşirli, N., Kaolen Yüzeyine Polyviniylimidazole Adsorpsiyonu, I. Ulusal Polimer Bilim ve Teknoloji Kongresi, Haziran 2006, Gazi Üniv., Ankara [11] Tekin, N., Dinçer, A., Demirbaş, Ö., and Alkan, M., Adsorpsiyo of Cationic Polyacrylamide on to Sepiolite, XX. Ulusal Kimya Kongresi, 2006, Erciyes Üniv., Kayseri [12] Alkan, M., Doğan, M., Demirbaş, Ö., Özdemir, Y., Sulu Çözeltilerden Sepiyolit

7 Yüzeyine Maxilon Yellow GL'nin Adsorpsiyon Kinetiği, XX. Ulusal Kimya Kongresi, 2006, Erciyes Üniv., Kayseri [13] Doğan, M., Türkyılmaz, A., Alkan, M., Demirbaş, Ö., Sepiyolit Yüzeyinde Bakır İyonlarının Adsorpsiyonu, XX. Ulusal Kimya Kongresi, 2006, Erciyes Üniv., Kayseri [14] Alkan, M., Kalay, B., Doğan, M., Demirbaş, Ö., Sulu Çözeltilerden Kaolinit yüzeyine Bakır Adsorpsiyonu, XX. Ulusal Kimya Kongresi, 2006, Erciyes Üniv., Kayseri [15] Doğan, M., Demirbaş, Ö., Özdemir, Y., Alkan, M., Namlı, H., 3- (Trimetoksisilil)Propilmetakrilat ile Modifiye Edilmiş Sepiyolit Örnekleri İle Sulu Çözeltilerden Metal İyonlarının Giderimi, XX. Ulusal Kimya Kongresi, 2006, Erciyes Üniv., Kayseri

8 ABSTRACT OF PhD THESIS IMMOBILIZATION OF SOME BIOMOLECULES ONTO CLAY MINERALS AND ELECTROKINETIC PROPERTIES Ozkan DEMIRBAS Balikesir University Institute of Natural and Applied Science Department of Chemistry PhD Thesis Supervisors: Prof. Dr. Mahir ALKAN, Assoc. Prof. Dr. Mehmet Dogan Balikesir, 2006 The presented thesis has been experimentally studied in three parts. The first part is a systematically investigation of the electrokinetic properties of some oxide minerals such as sepiolite, kaolinite, and expanded and unexpanded perlites. The second part is immobilization of some biomolecules such as bovine serum albumin, casein, catalase and lysozyme, and electrokinetic properties of biomolecules-covered particles finally the last part is the studying the enzymatic activity of pure and immobilized enzyme and calculation of kinetic parameters such as Km and Vmax. The study of the electrochemical properties of the oxide/water interface is important for understanding a large number of properties of oxide-rich porous media and colloid suspension stability of oxides. For this purpose, in first section of this study, the electrokinetic properties of some oxide minerals have been investigated using the microelectrophoresis technique as a function of solid/liquid ratio, ph and type and concentration of electrolyte. The electrokinetic properties of clay suspensions have been determined in aqueous solutions of mono-, di-, and trivalent salts such as NaCl, KCl, LiCl, NaNO3, NaCH3COO, MgCl2, CaCl2, BaCl2, Na2CO3, Na2SO4, AlCl3, FeCl3, Na3PO4 and divalent heavy metal salts such as CoCl2, CuCl2 and Pb(NO3)2. Furthermore, systematic zeta potential experiments have been performed to determine the isoelectric point (iep) and potential determining ions (pdi). From the experimental results, (i) the zeta potential of clays is not significantly affected by the solid concentration; (ii) ph strongly altered the zeta potential of clays; (iii) sepiolite and kaolinite have an isoelectric point at about ph 2.35 and 6.60 respectively whereas perlite samples has no iep at studied ph ranges, (iv) monovalent cations and anions, and di- and trivalent anions were indifferent electrolytes for clays whereas MgCl2, CaCl2, BaCl2, CoCl2, CuCl2, Pb(NO3)2, AlCl3 and FeCl3 changed the interface charge from negative to positive for sepiolite, kaolinite and expanded perlite but the potential determining ions for unexpanded perlite are CaCl2, BaCl2, AlCl3, and FeCl3 metal ions.the adsorption properties of biomolecules such as, bovine serum albumin, casein, catalase and lysozyme onto some oxide minerals were studied as a function of concentrations of biomolecules, sodium phosphate and NaCl concentration, ph and temperature of the solution. According to the experimental datas, adsorption capacity values showed a great dependence on ph. It was found that qe-ph curves reached a maximum at around isoelectric point (iep) of biomolecules. The ph values where the maximum adsorbed mass occurred might be considered as the conditions where electrostatic attraction is the most favourable. Both structural and electrostatic effects must be invoked to explain the diminution of adsorbed biomolecules on either side of the iep. Structural effects were related to the different conformational states that biomolecules adopted the changes with ph. Furthermore, the electrokinetic properties of biomolecules-covered oxide particles were also investigated. Zeta potentials of the minerals were also measured as a function of different biomolecules and phosphate concentrations, ph and ionic strength of solution indicated by NaCl concentration.

9 As a last part of the study, the activities of pure and immobilized enzymes were determined spectrophotometrically by the direct measurement of the decrease of light absorption caused by the decomposition of substrates. The Km and Vmax values were calculated by means of the Lineweaver Burke plot. Keywords: Clay mineral; protein; enzyme; immobilization; adsorption; zeta potential; enzyme activity.