EK-4 ÖZGEÇMİŞ 1. Adı Soyadı: Meltem Müftüoğlu 2. Unvanı: Profesör 3. Öğrenim Durumu: Derece Alan Üniversite Yıl Lisans Biyoloji Orta Doğu Teknik Üniversitesi 1991 Yüksek Lisans Hacettepe Üniversitesi 1995 Doktora Hacettepe Üniversitesi 2001 4. Akademik Unvanlar Yardımcı Doçent 2001 (Hacettepe Üniversitesi, Tıp Fakültesi, Anabilim Dalı) Doçent 2005 ( - YÖK) Profesör 2015 Görev Yaptığı Üniversite ve Araştırma Enstitüleri 1. Hacettepe Üniversitesi, Tıp Fakültesi, Anabilim Dalı, Ankara (1993-2004) 2. National Institute of Health, National Institute on Aging, Laboratory of Molecular Genetics, USA (04/1999-06/2000) 3. National Institutes of Health, National Institute on Aging, Laboratory of Molecular Gerontology, USA (2003-2009) 4. University of Copenhagen, Faculty of Medicine, Department of Cellular and Molecular Medicine, Center for Healthy Aging, Copenhagen, Denmark (2009-2011) 5. Koç Üniversitesi, Tıp Fakültesi, Tıbbi Anabilim Dalı, Istanbul (2011-07/2013) 6. Acıbadem Üniversitesi, Biyoloji ve Genetik Bölümü, Istanbul (2014- ) 5. Yönetilen Yüksek Lisans ve Doktora Tezleri 5.1 Yüksek Lisans Tezleri Tamamlanmış 1- Arın Doğan "İnsan Cockayne sendromu B proteininin NEIL1 enzim aktiviteleri üzerine etkilerinin araştırılması", Hacettepe Üniversitesi-2008 (Eş Danışman). 2- Derya Aydın "Bitki tabanlı küçük molekül inhbitörlerinin kanser türleri üzerinde potansiyel ekilerinin araştırılması", Koç Üniversitesi-2012 (Eş Danışman) 3- İlgü Ece Gülser DNA polimeraz gama ya bağlanan proteinlerin belirlenmesi ve kanser üzerine etkilerinin araştırılması, Acıbadem Üniversitesi- 2015 (Danışman).
6. Yayınlar 6.1 Uluslararası hakemli dergilerde yayınlanan makaleler 1. Ozer N, Muftuoglu M, and Ogus IH. A simple and sensitive method for the activity staining of xanthine oxidase. J Biochem Biophys Methods, 36, 95-100, 1998. 2. Ozer N, Muftuoglu M, Ataman D, Ercan A, and Ogus IH. Simple, high-yield purification of xanthine oxidase from bovine milk. J Biochem Biophys Methods, 39, 153-159, 1999. 3. Tuo J, Muftuoglu M, Chen C, Jaruga P, Selzer R, Brosh R, Rodriquez H, Dizdaroglu M, and Bohr VA. The Cockayne syndrome group B gene product is involved in general genome base excision repair of 8-hydroxyguanine in DNA. J Biol Chem, 276, 45772-45779, 2001. 4. Muftuoglu M, Selzer RR, Tuo J, Brosh RM Jr, and Bohr, V.A. Phenotypic consequences of mutations in the conserved motifs of the putative helicase domain of the human Cockayne syndrome group B gene. Gene, 283, 27-40, 2002. 5. Selzer RR, Nyaga S, Tuo J, May A, Muftuoglu M, Christiansen M, Citterio E, Brosh RM Jr., and Bohr VA. Differential requirement for the ATPase domain of the Cockayne syndrome group B gene in the processing of UV-induced DNA damage and 8-oxoguanine lesions in human cells. Nucleic Acids Res, 30, 782-93, 2002. 6. Muftuoglu M, Dalmizrak O, Elibol B, Ercan A, Kulaksiz G, Ogus H, Dalkara T, and Ozer N. Mitochondrial complex I and IV dysfunction of leukocytes in Parkinson`s Disease. Turk J Biochem, 28, 246-251, 2003. 7. Muftuoglu M, Elibol B, Dalmizrak O, Ercan A, Kulaksiz G, Ogus H, Dalkara T, and Ozer N. Mitochondrial complex I and IV activities in leukocytes from patients with parkin mutations. Movement Disorders, 19, 544-548, 2003. 8. Muftuoglu M, Wong K, Imam S, Wilson DM, Bohr VA, and Opresko PL. Telomeric protein TRF2 interacts with base excision repair proteins and stimulates DNA synthesis by DNA polymerase β. Cancer Research, 66(1),113-24, 2006. 9. Muftuoglu M, Sharma S, Thorslund T, Stevnsner T, Soerensen MM, Brosh RM and Bohr VA. Cockayne syndrome group B protein has novel strand annealing and exchange activities. Nucleic Acids Res, 34(1), 295-304, 2006. 10. Cheng WH, Muftuoglu M, and Bohr VA. Werner syndrome protein takes control of DNA strand breaks in S-phase. Exp Gerontol, 42(9), 871-878, 2007. 11. Kusumoto R, Muftuoglu M, and Bohr VA. The role of WRN in DNA repair is affected by post-translational modifications. Mech Aging Dev, 128(1), 50-57, 2007. 12. Wong HK, Muftuoglu M, Beck G, Imam SY, Bohr VA and Wilson DM. Cockayne syndrome B protein stimulates apurinic endonuclease I activity and protects against agents that introduce base excision repair intermediates. Nucleic Acids Res, 35(12), 4103-13, 2007. 13. Muftuoglu M, Kulikowicz T, Beck G, Lee JW, Piotrowski J, and Bohr VA. Intrinsic ssdna annealing activity in the C-terminal region of WRN. Biochemistry, 47(39), 10247-10254, 2008. 14. Cheng WH, Muftic D, Muftuoglu M, Dawut L, Morris C, Helleday T, Shiloh Y, and Bohr VA. WRN is required for ATM activation and the S-phase checkpoint in response to interstrand cross-link-induced DNA double-strand breaks. Mol Biol Cell, 19, 3923-3933, 2008.
15. Muftuoglu M, Oshima J, von Kobbe C, Cheng WH, Leistritz DF, and Bohr VA. The clinical characteristics of Werner Syndrome: molecular and biochemical diagnosis. Human Genet, 124, 369-377, 2008. 16. Stevnsner T, Muftuoglu M, Aamann MD, and Bohr VA. The role of Cockayne Syndrome group B (CSB) protein in base excision repair and aging. Mech Ageing Dev, 129 (7-8), 441-448, 2008. 17. Muftuoglu M, Kusumoto R, Speina E, Beck G, Cheng WH and Bohr VA. Acetylation regulates WRN catalytic activities and affects base excision repair. PLoS ONE, 3(4):1-14, 2008. 18. Ercan A, Kulaksiz G, Dalmizrak O, Muftuoglu M, Ogus H, Cavdar L, Inan L, and Ozer N. Mitochondrial respiratory chain enzyme activities, mtdna variants and gene expression levels in Idiopathic Parkinson s Disease. Turk J Biochem, 34, 97-104, 2009. 19. Muftuoglu M, de Souza-Pinto NC, Dogan A, Aamann M, Stevsner T, Rybanska I, Kirkali G, Dizdaroglu M and Bohr VA. Cockayne syndrome group B protein stimulates repair of formamidopyrimidines by NEIL1 DNA glycosylase. J Biol Chem, 284(14), 9270-79, 2009. 20. De Souza-Pinto NC, Maynard S, Hashiguchi K, Hu J, Muftuoglu M, and Bohr VA The recombination protein RAD52 cooperates with the excision repair protein OGG1 for the repair of 8-hydroxyguanine lesions. Mol Cell Biol, 29(16), 4441-54, 2009. 21. Aaman M, Sorensen M, Hvitby C, Berquist B, Muftuoglu M, Tian J, De Souza Pinto N, Scheibye-Knudsen M, Wilson D, Stevnsner T, and Bohr VA. Cockayne syndrome group B protein promotes mitochondrial DNA stability by supporting the DNA repair association with the mitochondrial membrane. FASEB J, 24, 1-13, 2010. 22. Aamann MD, Muftuoglu M, Bohr VA, Stevnsner T. Multiple interaction partners for Cockayne syndrome proteins: Implications for genome and transcriptome maintenance. Mech Ageing Dev, 134 (5-6), 212-24, 2013. 23. Aamann MD, Hvitby C., Popuri V, Muftuoglu M, Lemminger L, Skeby C, Keijzers G, Ahn B, Bjørås M, Bohr VA, and Stevnsner T. Cockayne Syndrome group B protein stimulates NEIL2 DNA glycosylase activity. Mech Ageing Dev, 135, 1-14, 2014. 24. Muftuoglu M, Mori MP, de Souza Pinto NC. Formation and repair of oxidative damage in the mitochondrial DNA. Mitochondrion, 17, 164-181, 2014. 6.2 Uluslararası bilimsel toplantılarda sunulan ve bildiri kitabında (Proceedings) basılan bildiriler (SCI kapsamında) 1. Dalmizrak O, Muftuoglu M, Ogus IH, and Ozer N. The Effects of Glutathione Depletion on Complex I Activity and Mitochondrial DNA in the Human Leukocytes, European Journal of Biochemistry, 269 (S1), 44, PS2-001, 2002. 2. Muftuoglu M, Selzer RR, Tuo J, Brosh RM Jr, and Bohr VA. Phenotypic consequences of mutations in the conserved motifs of the putative helicase domain of the human Cockayne syndrome group B gene. European Journal of Biochemistry, 269 (S1), 41, PS1-024, 2002. 3. Dalmizrak O, Muftuoglu M, Atac B, Ogus IH, Ozer N and Elibol B. Mitochondrial Complex I and Complex IV Activities in Lymphocytes from Patients with Parkin Gene Mutations, Movement Disorders, 17 (S5), S62-S63, P164, 2002. 4. Opresko Pl., Muftuoglu M., Mason P., Wilson DM., and Bohr VA. RECQ helicases and DNA repair pathways at telomeric DNA. Mutation
Research/Fundamental and Molecular Mechanisms of Mutagenesis, 577 (S1), 356, 2005. 5. Muftuoglu M., Kusumoto R., von Kobbe C., and Bohr VA. The Functional Consequences of Acetylation on WRN protein. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 577 (S1), 345, 2005. 6. Muftuoglu M., Opresko P., and Bohr VA. Base excision repair pathway at telomeric DNA. The FEBS Journal, 273 (S1), 54, OP-41, 2006. 7. Ercan A., Kulaksiz G., Muftuoglu M., Dalmizrak O., Ogus H, Cavdar L., Inan L., and Ozer N. Mitochondrial complex I and IV activities, ND2, ND4 gene mutations and expressions in idiopathic Parkinson`s disease. The FEBS Journal, 273 (S1), 244-245, PP-622, 2006. 8. Muftuoglu M,. Aamann MD, Stevnsner T, Bohr VA. The involvement of Cockayne syndrome B protein in Base Excision Repair. The FEBS Journal, 280 (S1), SW01.S3-29, 2013. 9. Oktem O, Muftuoglu M, Senbabaoglu F, Urman B. Real-time analysis of the growth of human granulosa cells using an impedance-based signal processing system: a new technology for translational research in human reproduction. Human Reproduction, 28 (S1), i54-55, O-128, 2013. 10. Oktem O, Muftuoglu M, Senbabaoglu F, and Urman B. Real-time and quantitaive assessment of the effects of different ovarian stimulation protocols on the proliferation and cell cycle kinetics of granulosa cells using a new impedencebased system. Fertility and Sterility, 100 (3), S260, 2013. 11. Oktem O, Muftuoglu M, Senbabaoglu F, and Urman B. Effect of pharmacologic inhibition of c-jun N-terminal kinase (JNK) signaling pathway on cell proliferation and cell cycle progression in human granulosa cell tumor. J Clinical. Oncology, 31 (15), S e22004, 2013. 12. Pamukcu C, Keskin N, Gulser IE, Erman B, Erman B, Uren A, Yakicier C, Muftuoglu M. Identification and characterization of small molecule inhibitors targeting DNA polymerase gamma for the treatment of cancers deficient in mismatch repair. The FEBS Journal, 282, S1, 115-116, 2015. Uluslararası bilimsel toplantılarda sunulan ve bildiri kitabında basılan bildiriler 1. Muftuoglu M, Dalmizrak O, Elibol B, Ercan A, Kulaksiz G, Ogus IH, Dalkara T, and Ozer N. Mitochondrial enzyme activities and mtdna polymorphisms in Parkinson`s disease. 13 th Balkan Biochemical Biophysical Days and Meeting on Metabolic Disorders, 28 (3), p.78, OP-3, Kusadası, Izmir, Turkey, 2003. 2. Opresko PL, Muftuoglu M, Wilson DM, and Bohr VA. Telomeric protein TRF2 stimulations DNA synthesis by DNA Polymerase β. Gordon Conference on DNA Damage, Mutations and Cancer, Venture, CA, 2005. 3. Beck G, Lee JW, Muftuoglu M, and Bohr VA. Mapping of the Werner helicase single strand annealing activity. 2nd Baltimore Area Repair Symposium: DNA damage and repair in Cancer, Baltimore, USA, 2006. 4. Hashiguchi K, Hu J, Maynard S, Muftuoglu M, Souza-Pinto N, and Bohr VA. OGG1 and RAD52 cooperate to repair oxidative DNA damage. 2nd Baltimore Area Repair Symposium: DNA damage and repair in Cancer, Baltimore, USA, 2006.
5. Wong HK, Muftuoglu M, Beck G, May A, and Bohr VA. Cooperation of Ape1 and CSB in repair of abasic sites. 2nd Baltimore Area Repair Symposium: DNA damage and repair in Cancer, Baltimore, USA, 2006. 6. Cheng W, Muftic D, Muftuoglu M, Helleday T, Shiloh Y, and Bohr VA. S-phase checkpoint mediated by WRN-dependent ATM activation. 2006 Internationsl Workshop on Ataxia-Telangiectasia (A-T) and ATM, P17, Oral presentation, Banff, Canada, 2006. 7. Bohr VA, Opresko P, Mason P, Muftuoglu M, Kusumoto R, and Cheng W. Werner and DNA repair at the telomere end. RecQ helicases and other helicases in telomere maintenance and related pathways. International workshop, Lansdowne, VA, USA, 2006. 8. Muftuoglu M, Opresko P, and Bohr VA. TRF2 stimulates polymerase beta DNA synthesis at telomeric and nontelomeric substrates. RecQ helicases and other helicases in telomere maintenance and related pathways. International workshop, Lansdowne, VA, USA, 2006. 9. Aydın D, Muftuoglu M and Erman B. Identifying small molecule inhibitors targeting base excision repair enzymes DNA polymerase gamma and beta to increase the effect of cancer treatment RECOMB 2012, Barcelona, Spain, 2012. 10. Guven Maiorov E, Keskin O, Muftuoglu M, Gursoy A. Designing and characterizing small molecule inhibitor(s) for mitochondrial transcription factor A (TFAM) for therapeutic benefit of Cancer. International Conference and Exhibition on Computer Aided Drug Design & QSAR. October 29-31, 2012, Chicago-North Shore, USA. 6.3 Yazılan uluslararası kitaplar veya kitaplarda bölümler 1. Ogus I H, Balk M, Aksoy Y, Muftuoglu M, and Ozer N. The effects of oxidative stress on the redox system of the human erythrocytes. Free radicals, oxidative stress and antioxidants, Pathological and physiological significance preceedings, NATO ASI Series, Plenum Publishing Corporation, New York 25-37, 1998. 2. Muftuoglu M and Bohr VA. Roles of Cockayne syndrome group B protein in processing oxidative DNA damage and in protection against neurodegeneration. Molecular Mechanisms of Cockayne Syndrome, Landes Bioscience, Texas, USA, July 22, 53-63, 2009. 3. Cheng WH, Muftuoglu M, Wu RTY. Selenium and epigenetic effects on histone marks and DNA methylation. Nutrition and Epigenetics. CRC Press, Taylor and Francis Group, New York USA. Chp 10, 273-299, 2014. 4. Oktay Y, Agirbasli D, Dalva-Aydemir S, Akyerli-Boylu C, Muftuoglu M, Yakicier C. Medical Genetics, Current Applications of Biotechnology. Chp 19, 299-317, 2014. 6.4 Ulusal hakemli dergilerde yayınlanan makaleler 1. Muftuoglu M and Ozer N. The in vitro effects of the antimalarial drug primaquine, on the activities of some enzymes in human erythrocytes lyzates. Turk J Med Sciences, 29, 409-412, 1999. 2. Muftuoglu M, DNA tamiri ve erken yaşlanma sendromları. Turk Dergisi, 28 (1), 20-24, 2003.
6.5 Ulusal bilimsel toplantılarda sunulan ve bildiri kitabında basılan bildiriler 1. Muftuoglu M and Ozer N. The in vitro effects of the antimalarial drug primaquine, on the activities of catalase, superoxide dismutase, and glucose 6-phosphate dehydrogenase in human erythrocytes. XIII. National Biochemistry Congress, (with international participation)/ B07, Turkey, 1996. 2. Ozer N, Muftuoglu M, Ataman D, Ercan A, and Ogus IH. A new and simple method for the purification of xanthine oxidase from cow milk, XIV. National Congress of Biochemistry and Symposium on Automation in Clinical Laboratory, (with international participation)/ B206, Turkey, 1997 3. Muftuoglu M and Ozer N. The in vitro effects of the antimalarial drug primaquine, on the activities of some enzymes in human erythrocyte lyzates. XIV. National Congress of Biochemistry and Symposium on Automation in Clinical Laboratory, (with international participation)/ C335, Turkey, 1997. 4. Ozer N, Muftuoglu M, and Ogus IH. A simple and sensitive method for the activity staining of xanthine oxidase on polyacrylamide gels. XIV. National Congress of Biochemistry and Symposium on Automation in Clinical Laboratory, (with international participation)/ C112, Turkey, 1997. 5. Muftuoglu M, Dalmizrak O, Ogus IH, and Ozer N. The effects of oxidative agents on mitochondrial heteroplasmy and electron transport chain complex I activity. 1 st National Molecular Genetic in Diagnosis Congress. P28, Adana, Turkey, 2002. 6. Dalmizrak O, Kulaksiz G, Ercan A, Muftuoglu M, Ogus IH, Cavdar L, Inan L, Terzioglu A, and Ozer N. The evaluation of mitochondrial complex I and IV enzyme activities in muscle biopsies of patients with idiopathic PD. National Congress of Biochemistry, 29 (1), 38-39, SB37, Trabzon, Turkey, 2004. 6.6 Diğer yayınlar Yazılan ulusal kitap bölümü Ozer N and Muftuoglu M. Gen Yapısı ve Bilgi İçeriği. Biyoinformatik- I Lisansüstü Yaz Okulu Kitabı, 41-50, 2003. Kitap bölümü çevirileri 1. The Art of Medicine (Anderson J, Barnes E, Shackleton, welcome collection), Hiber Tıp Yayınları, 2012. 2. Biochemistry textbook (Stryer L; Seventh ed. 2010), Chp 29, RNA synthesis and Processing, Palme yayınevi, Ankara, 2013. Atıflar Google Scholar a göre: Atıflar: 1053, h-endeksi: 18 7. Projeler 1. Oksidatif ajanlarin Memeli Mitokondri DNA`si Üzerindeki Etkileri ve Bu etkilerin Mitokondriyel Heteroplazmi ve Yaslanma ile Iliskilerinin Arastirilmasi. TUBITAK projesi, proje no: TBAG-1754, Araştırmacı, 2002. 2. Parkinson Hastaliginda Mitokondriyel DNA mutasyonlarinin belirlenmesi. Hacettepe Universitesi, Bilimsel Arastirmalar Birimi Projesi. Proje no. 0002101001, Araştırmacı, 2002.
3. Parkinsonlu Hastalarda Mitokondriyel ND2 ve ND4 Mutasyonlarinin ve Gen Expresyon Duzeylerinin Saptanarak Kompleks I aktivitesi ile ilişkisinin Arastirilmasi. TUBITAK Projesi. Proje No. SBAG- 2596, Yardımcı Yürütücü, 2004. 4. Idiyopatik Parkinson Hastalarinin Kas Dokusunda, Mitokondriyel Solunum Zinciri Enzim Duzeylerinin ve mtdna Mutasyonlarinin Belirlenmesi. Hacettepe Universitesi, Bilimsel Arastirmalar Birimi Projesi. Proje no. 02G112, Araştırmacı, 2005. 5. SIRT1 mediated protection of mitochondrial biogenesis in Cockayne syndrome B protein deficient cells upon oxidative stress. Nordea Foundation, Kopenhag, Danimarka, Proje no: 29068, Proje Yürütücüsü, 2009-2011. 6. Özgün İlaç Keşfi, İlaç Geliştirilmesi ve İstanbul İlaç Sektörü ne Bilgi ve Teknoloji Aktarma Amaçlı Bir-Teknoloji Merkezi ve Üniversite-Sanayi İşbirliği Ağının Kurulması. Istanbul Kalkınma Ajansı (ISTKA) projesi, BIL123, Ortak, 2013-2014. 7. Kanser tedavisine yönelik DNA polimeraz gamma enzimini hedefleyerek mitokondriyel baz eksizyon tamir mekanizmasını bloke eden küçük molekül inhibitörlerinin belirlenmesi ve etkinliklerinin saptanması. TÜBİTAK 1001 projesi, TBAG:212T026, Proje yürütücüsü, 2014-2016. 8. Sporadik Alzheimer hastalığı için yeni bir genetik risk faktörü olarak baz eksizyon tamir genlerinin genetik varyantlarının taranması ve değerlendirilmesi. TUBİTAK 1001 projesi, KBAG114Z875, Proje yürütücüsü, 2015-2018. 9. Özgün 2-indolinon bileşiklerinin anti-interlökin 1 ve kemoterapötik ilaçlar olarak geliştirilmesi/dna tamir inhibitörlerinin polipsiz kalıtsal kolon kanseri (MLH1 gen defekti) tedavisi için yeni bir ilaç olarak geliştirilmesi. TÜBİTAK 1003, Proje Yürütücüsü, 2015-2018. 8. İdari Görevler Fen-Edebiyat Fakültesi Dekan Yardımcısı Erasmus Fakülte Koordinatörü 9. Bilimsel Kuruluşlara Üyelikler 1. Türk Derneği 2. Federation of European Biochemical Societies 3. DNA Repair Interest Group 4. Mitochondria Interest Group 10. Ödüller 1. NIH visiting fellowship, Ruth L. Kirschstein National Research Service Award (NRSA) (2004-2009) 2. International Academy of Achievement Summit (2005) 3. NIH Fogarty scholarship (09/1999-06/2000) 4. TUBITAK NATO A2 bursu (04-08/1999)
11. Son iki yılda verdiğiniz lisans ve lisansüstü düzeydeki dersler için aşağıdaki tabloyu doldurunuz. Lisans düzeydeki dersler Akademik Dönem Bölüm Yıl 2012-2013 Güz Koç Üniversitesi Tıp Fakültesi Dersin Adı Dönem II, 1. Ders Kurulu, MEDI201 Dönem II, 2. Ders Kurulu, MSKL202 Dönem II, 3. Ders Kurulu, BLDI203 Toplam saat Öğrenci Teorik Uygulama Sayısı 17 8 32 16-32 12 8 32 İlkbahar Koç Üniversitesi Tıp Fakültesi Dönem II, 5. Ders Kurulu, NUTI205 Dönem II, 6. Ders Kurulu, ERUS206, 12 3 32 17-32 2014-2015 2014-2015 Güz İlkbahar Acıbadem Üniversitesi, Biyoloji ve Genetik Bölümü Acıbadem Üniversitesi, Biyoloji ve Genetik Bölümü MBG201-Hücre Biyolojisi I MBG202- Hücre Biyolojisi II 42 28 4 42 28 4 Yüksek lisans düzeydeki dersler Dönem Bölüm Akademik Yıl 2012-2013 İlkbahar 2014-2015 İlkbahar Koç Üniversitesi Sağlık Bilimleri Enstitüsü Acıbadem Üniversitesi, ve Biyoloji Lisansüstü Programı Dersin Adı da özel konular (HSBM453) BMB-627-DNA Onarımı ve Mutagenezi Toplam saat Teorik Uygulama Öğrenci Sayısı 13 130 1 42-2